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Prolactin ELISA

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Human prolactin (lactogenic hormone) is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin is secreted from the anterior pituitary gland in both men and woman. Women normally have slightly higher basal prolactin levels than men. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications. Hyperprolactinemia inhibits hypogonadism in men and women with accompanying low FSH and LH levels. The Prolactin blood test is used to measure for irregularities.

Catalog number
Product type ELISA
96 Tests (12x8 breakable strip wells)
Standard range 5-100 ng/ml
Analytical Sensitivity
5 ng/ml
Sample volume 25 µl/well
Species Human
Application The Calbiotech Prolactin ELISA kit is intended for the quantitative measurement of prolactin in human serum.
The Calbiotech Prolactin ELISA kit is a solid phase sandwich ELISA assay method, based on a streptavidin-biotin principle. The standards, samples and a reagent mixture of Anti-Prolactin Enzyme and Biotin conjugates are added into the wells, coated with Streptavidin. Prolactin in the patient's serum forms a sandwich between two highly specific Prolactin antibodies, labeled with Biotin and HRP. Simultaneously, the biotinylated antibody is immobilized onto the well through a high affinity Streptavidin-Biotin interaction. Unbound protein and excess biotin/enzyme conjugated reagent are washed off by wash buffer. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of Prolactin in the samples. A standard curve is prepared relating color intensity to the concentration of the Prolactin.
Storage and Stability Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.
Precautions For research use only. Not for use in diagnostic procedures.

1. Vanderpump MP; French JM; Appleton D; Tunbridge WM; Kendall-Taylor P. The prevalence of hyperprolactinaemia and association with markers of autoimmune thyroid disease in survivors of the Whickham Survey cohort. Clin Endocrinol (Oxf) 1998; 48(1):39-44. 

2. Straub RH; Zeuner M; Lock G; Sch¨olmerich J; Lang B. High prolactin and low dehydroepiandrosterone sulphate serum levels in patients with severe systemic sclerosis. Br J Rheumatol 1997; 36(4):426-32. 

3. Neidhart M. Elevated serum prolactin or elevated prolactin/cortisol ratio are associated with autoimmune processes in systemic lupus erythematosus and other connective tissue diseases. J Rheumatol 1996; 23(3):476-81. 

4. Neidhart M Serum levels of interleukin-1 beta, luteinizing hormone, and prolactin correlate with the expression of CD45 isoforms on CD4+ peripheral blood T lymphocytes in healthy women. Ann Hematol 1997; 75(4):155-9. 

5. Maes M; Mommen K; Hendrickx D; Peeters D; D'Hondt P; Ranjan R; De Meyer F; Scharp´e S. Components of biological variation, including seasonality, in blood concentrations of TSH, TT3, FT4, PRL, cortisol and testosterone in healthy volunteers. Clin Endocrinol (Oxf) 1997; 46(5):587-98. 


Prolactin Package Insert
Type : PDF

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