||96 Tests (12x8 breakable strip wells)|
|Standard range||0.25-7.5 ng/ml|
|Sample volume||25 µl/well|
|Species||Mouse or Rat|
|Application||The Calbiotech Mouse/Rat Triiodothyronine (T3) ELISA Kit is intended for the quantitative measurement of Triiodothyronine (T3) in mouse/rat serum or plasma.|
The Calbiotech Mouse/Rat T3 is a solid phase competitive ELISA. The samples, the working T3 enzyme conjugate, diluted in assay diluent, are added to the wells coated with anti-T3 polyclonal antibody. T3 in the patient’s serum competes with T3 enzyme conjugate for binding sites.
Unbound T3 and T3 enzyme conjugate is washed off by washing buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T3 in the samples. A standard curve is prepared relating color intensity to the concentration of the T3.
|Storage and Stability||Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.|
|Precautions||For research use only. Not for use in diagnostic procedures.|
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