||96 Tests (12x8 breakable strip wells)|
|Standard range||3-300 pg/ml|
|Sample volume||25 µl/well|
|Species||Mouse or Rat|
|Application||The Calbiotech Mouse/Rat Estradiol (E2) ELISA Kit is intended for the quantitative determination of Estradiol (E2) concentration in Mouse or Rat serum and plasma.|
The Calbiotech Mouse/Rat E2 ELISA kit is based on the principle of competitive binding between E2 in the test specimen and E2 enzyme conjugate for a constant amount of anti-Estradiol polyclonal antibody. In the incubation, anti-E2 antibody coated wells are incubated with E2 standards, controls, samples, and E2 enzyme conjugate at room temperature for 120 minutes with. During the incubation, a fixed amount of HRP-labeled E2 competes with the endogenous E2 in the standard, sample, or quality control serum for a fixed number of binding sites of the specific E2 antibody. E2 peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of E2 in the specimen increases. Unbound E2 peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. A standard curve is obtained by plotting the concentration of the standard versus the absorbance.
|Storage and Stability||Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.|
|Precautions||For research use only. Not for use in diagnostic procedures.|
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