||96 Tests (12x8 breakable strip wells)|
|Standard range||5-100 ng/ml|
|Sample volume||10 µl/well|
|Species||Mouse or Rat|
|Application||The Calbiotech Cotinine Direct ELISA Kit is intended for the measurement of Cotinine in Mouse or Rat serum or urine.|
The Calbiotech Cotinine kit is a solid phase competitive ELISA. The samples and Cotinine enzyme conjugate are added to the wells coated with anti-Cotinine antibody. Cotinine in the samples competes with a Cotinine enzyme (HRP) conjugate for binding sites. Unbound Cotinine and Cotinine enzyme conjugate is washed off by washing step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cotinine in the samples. A standard curve is prepared relating color intensity to the concentration of the Cotinine.
|Storage and Stability||Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.|
|Precautions||For research use only. Not for use in diagnostic procedures.|