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Canine Fentanyl ELISA

SKU: FE087D-300
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Product type ELISA
96 Tests (12x8 breakable strip wells)
Sample volume 20 µl/well
Species Canine
Application The Calbiotech Canine Fentanyl Direct ELISA Kit is a sensitive in-vitro test to detect the presence of Fentanyl in Canine serum or urine.
The Calbiotech Canine Fentanyl Direct ELISA Kit is based upon the competitive binding to antibody of enzyme labeled antigen and unlabeled antigen, in proportion to their concentration in the reaction mixture. A 20 μl. aliquot of a diluted unknown specimen is incubated with a 100 μl. dilution of enzyme (Horseradish peroxidase) labeled Fentanyl derivative in micro-plate wells, coated with fixed amounts of high affinity purified polyclonal anti-Fentanyl. The wells are washed thoroughly and a chromogenic substrate added. The color produced is stopped using a dilute acid stop solution and the wells read at 450 nm. The intensity of the color developed is inversely proportional to the concentration of drug in the sample. The technique is sensitive to 0.1 ng/ml. The Canine Fentanyl Direct ELISA Kit avoids extraction of urine or blood sample for measurement. It employs a Fentanyl directed antiserum. Due to the proprietary method of orienting the antibody on the polystyrene micro-plate much higher sensitivity is achieved compared to passive adsorption. This allows an extremely small sample size, reducing matrix effects and interference with binding proteins(s) or other macromolecules.
Storage and Stability Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.
Precautions For research use only. Not for use in diagnostic procedures.
References 1. R.C. Baselt, R.H. Cravey. Disposition of Toxic Drugs and Chemicals in Man. Chemical Toxicology
Institute, Foster City 319-321.

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