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Secretory IgA ELISA

SKU: SC221A
Price:
$450.00
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Catalog number
SC221A
Product type ELISA
Quantity                          
96 Tests (12x8 breakable strip wells)
Standard range 6.25-200 µlg/dl
Analytical Sensitivity
6.25 µlg/dl
Sample volume 10 µl/well
Species Human
Application The Calbiotech Secretory IgA (sIgA) ELISA Kit is intended for the quantitative measurement of sIgA in human stool or saliva.
Principle
The Calbiotech Secretory IgA (sIgA) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-sIgA monoclonal antibody, along with the incubation buffer. After a simple washing step, an anti-SIgA enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, a chromogenic substrate (TMB) is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of sIgA in the samples. A standard curve is generated relating color intensity to the concentration of sIgA.
Storage and Stability Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.
Precautions For research use only. Not for use in diagnostic procedures.
References

1. N.J. Mantis, et.al. (June 2011). "Secretory IgA's complex roles in immunity and mucosal homeostasis in the gut." Mucosal Immunology (2011) 4, 603–611; doi:10.1038/mi.2011.41;