||96 Tests (12x8 breakable strip wells)|
|Standard range||6.25-200 µlg/dl|
|Sample volume||10 µl/well|
|Application||The Calbiotech Secretory IgA (sIgA) ELISA Kit is intended for the quantitative measurement of sIgA in human stool or saliva.|
The Calbiotech Secretory IgA (sIgA) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-sIgA monoclonal antibody, along with the incubation buffer. After a simple washing step, an anti-SIgA enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, a chromogenic substrate (TMB) is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of sIgA in the samples. A standard curve is generated relating color intensity to the concentration of sIgA.
|Storage and Stability||Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.|
|Precautions||For research use only. Not for use in diagnostic procedures.|
1. N.J. Mantis, et.al. (June 2011). "Secretory IgA's complex roles in immunity and mucosal homeostasis in the gut." Mucosal Immunology (2011) 4, 603–611; doi:10.1038/mi.2011.41;