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Neuron Specific Enolase (NSE) ELISA

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Catalog number
Product type ELISA
96 Tests (12x8 breakable strip wells)
Standard range 5-150 ng/ml
Analytical Sensitivity
5 ng/ml
Sample volume 25 µl/well
Species Human
Application The Calbiotech NSE ELISA is intended for the quantitative measurement of NSE in human serum or plasma.
The Calbiotech NSE ELISA kit is a solid phase direct sandwich ELISA. The standards, samples and controls are added into the selected wells which are pre-coated with anti human NSE monoclonal anybody along with an anti-NSE-HRP conjugated pair match antibody. NSE, in the standards, controls and patient samples binds to anti-NSE antibody in the wells and anti-NSE-HRP conjugated antibody binds to the NSE. The unbound glycolytic enzyme enolase, NSE, is washed off by wash buffer. Upon the addition of the TMB substrate, the intensity of color developed is proportional to the concentration of NSE in the samples. A standard curve is prepared relating color intensity to the concentration of the NSE.
Storage and Stability Product should be stored at 2-8 °C. Product is stable for 24 months from the date of manufacturing.
Precautions For research use only. Not for use in diagnostic procedures.

1. F.J. Tapia;A.J.A. Barbosa; P.J. Marangos; J.M. Polak; S.R. Bloom; C. Dermody; A.G.E. Pearse. Neuron-Specific Enolase is produced by neuroendocrine tumours. The Lancet 1981; 317: 808-811 

2. E. Paus; K. Nustad: Immunoradiometric Assay for αγ- and γγ-Enolase (Neuron-Specific Enolase), with Use of Monoclonal Antibodies and Magnetizable Polymer Particles. Clin. Chem.1989, 35: 2034-2038. 

3. K. Iwase, A. Kato, et al. Enolase subunits in patients with neuroendocrine tumors. J Clin Endocrinol Metab 1986; 63:94-101. 

4. D. Schmechel, PJ Marangos, AP Zis, M. Brightman, FK. Goodwin. The brain enolase specific markers of neuronal and glia cells. Science 1978; 199:313-5 

5. P. Ellisabeth, N. Kjell. Immunoradiometric assay for the αγ and γγ –enolase (Neurons-Spacific Enolase), with use of monoclonal antibodies and magnetizable polymer particles. Clin. Chem. 1989, 35/10, 2034-2038