Methamphetamine ELISA

Category Name

Drugs of Abuse

Method

ELISA

Principle

ELISA

Controls Standards

0,10,25,50ng/ml

Sensitivity

1ng/ml

Sample

10 µl

Runtime

90 min

Wavelength

450 nm

Safety

Please refer to MSDS

Shelflife

18 Months

ME090D-R4

Methamphetamine MSDS

INTENDED USE

The Calbiotech, Inc. (CBI) Methamphetamine Direct ELISA Kit provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/ mass spectrometry (GS-MS) is the preferred confirmatory method. Professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.

SUMMARY AND EXPLANATION

The Methamphetamine Direct ELISA Kit is a specific and sensitive in-vitro test to detect the presence of d-methamphetamine in samples such as whole blood, oral fluids, serum, plasma and urine. While the assay will detect amphetamine use, interference by l-methamphetamine and pseudo-ephedrine is virtually nonexistent. Methamphetamine is a potent central nervous system stimulant with less peripheral actions than mphetamine. The (+)-isomer also referred to as d-methamphetamine is ten times more potent than the (-)-isomer, l-methamphetamine. Amphetamines act by inducing euphoria, irritability, anxiety and paranoia Methamphetamine is metabolized to its active metabolite amphetamine (via N-demethylation) and is further metabolized by hydroxylation and deamination of amphetamine. Urinary excretion rates are influenced by the urinary pH with acidic urine favoring the excretion of unchanged drug. Alkaline urine reduces the excretion of unchanged methamphetamine to less than 5% of the dose.

PRINCIPLES OF THE TEST
The Methamphetamine Direct ELISA Kit (for d-methamphetamine measurement) is based upon the competitive binding to antibody of enzyme labeled antigen and unlabeled antigen, in proportion to their concentration in the reaction mixture. A 10 ml. aliquot of a diluted unknown specimen is incubated with a 100 ml. dilution of enzyme (Horseradish peroxidase) labeled d-methamphetamine derivative in micro-plate wells, coated with fixed amounts of oriented high affinity purified polyclonal antibody. The wells are washed thoroughly and a chromogenic substrate added. The color produced is stopped using a dilute acid stop solution and the wells read at 450 nm. The intensity of the color developed is inversely proportional to the concentration of drug in the sample. The technique is sensitive to 1 ng/ml. The Methamphetamine Direct ELISA Kit avoids extraction of urine sample for measurement. It employs a d-methamphetamine directed antiserum. Due to the proprietary method of orienting the antibody on the polystyrene micro-plate much higher sensitivity is achieved compared to passive adsorption. This allows an extremely small sample size, reducing matrix effects and interference with binding proteins(s) or other macromolecules.