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PRESS RELEASE: Calbiotech awarded SBIR Grant!
Calbiotech has been awarded an SBIR Grant by U.S. Army to develop assays for Dengue Virus.
Calbiotech has been awarded an SBIR Grant by U.S. Army to develop assays for Dengue Virus.
Cannabinoids ELISA
Category Name
Drugs of Abuse
Method
ELISA
Principle
ELISA
Controls Standards
0,2,5,10ng/ml
Sensitivity
1ng/ml
Sample
10 µl
Runtime
90 min
Wavelength
450 nm
Safety
Please refer to MSDS
Shelflife
18 Months
INTENDED USE
The Calbiotech, Inc. (CBI) Cannabinoids Direct ELISA Kit provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography/ mass spectrometry (GS-MS) is the preferred confirmatory method (1). Professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.
EXPLANATION OF THE TEST
The Cannabinoids Direct ELISA Kit is a specific and sensitive in-vitro test to detect the presence of cannabinoids in samples such as whole blood, serum, plasma and urine. ƒ´9-THC (a member of the cannabinoid family) is the primary psychoactive ingredient of marijuana (1). Cannabinoid metabolites appear in urine two to four hours after a marijuana smoke and may persist for days (up to thirty) (1‑ 3). Thus a urine assay reasonably serves to detect cannabis use even though a considerable period may have elapsed since smoking or ingestion of marijuana.
PRINCIPLES OF THE PROCEDURE
The Cannabinoids Direct ELISA Kit is based upon the competitive binding to antibody of enzyme labeled antigen and unlabeled antigen, in proportion to their concentration in the reaction mixture. A 10 ml. aliquot of a diluted unknown specimen is incubated with a 100 ml. dilution of enzyme (Horseradish peroxidase) labeled carboxy THC (THCA) derivative in micro-plate wells, coated with fixed amounts of oriented high affinity purified polyclonal antibody. The wells are washed thoroughly and a chromogenic substrate added. The color produced is stopped using a dilute acid stop solution and the wells read at 450 nm. The intensity of the color developed is inversely proportional to the concentration of drug in the sample. The technique is sensitive to 1 ng/ml. The THC Direct ELISA Kit avoids extraction of urine or blood sample for measurement. It employs a polyclonal high affinity, purified carboxy THC antibody. Due to the proprietary method of orienting the antibody on the polystyrene micro-plate much higher sensitivity is achieved compared to passive adsorption. This results in extremely small sample size reducing matrix effects and interference with binding proteins(s) or other macromolecules.
The Cannabinoids Direct ELISA Kit is based upon the competitive binding to antibody of enzyme labeled antigen and unlabeled antigen, in proportion to their concentration in the reaction mixture. A 10 ml. aliquot of a diluted unknown specimen is incubated with a 100 ml. dilution of enzyme (Horseradish peroxidase) labeled carboxy THC (THCA) derivative in micro-plate wells, coated with fixed amounts of oriented high affinity purified polyclonal antibody. The wells are washed thoroughly and a chromogenic substrate added. The color produced is stopped using a dilute acid stop solution and the wells read at 450 nm. The intensity of the color developed is inversely proportional to the concentration of drug in the sample. The technique is sensitive to 1 ng/ml. The THC Direct ELISA Kit avoids extraction of urine or blood sample for measurement. It employs a polyclonal high affinity, purified carboxy THC antibody. Due to the proprietary method of orienting the antibody on the polystyrene micro-plate much higher sensitivity is achieved compared to passive adsorption. This results in extremely small sample size reducing matrix effects and interference with binding proteins(s) or other macromolecules.
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