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DHEA-S ELISA

Category Name
Steroids
Method
ELISA
Principle
ELISA
Controls Standards
0,0.1,0.5,1.0,2.5,5,10µg/ml
Sensitivity
0.02µg/ml
Sample
25 µl
Runtime
75 min
Wavelength
450 nm
Safety
Please refer to MSDS
Shelflife
18 Months

Item # DH079S $300 qty:

DHEA-S_ELISA_Package_Insert.pdf  DHEA-S ELISA Package Insert
DH079S-R2-MSDS.pdf  DHEA-S ELISA MSDS

INTENDED TO USE
The Calbiotech DHEA-S Kit is an ELISA for the measurement of DHEA-S in human serum or plasma. 
 
SUMMARY AND EXPLANATION
DHEA-S is a more specific product of the adrenals and measurements of this steroid are widely intended in clinical practice. The clinical importance of plasma assays of DHEA-S is associated with the diagnosis of adrenal hyperplasia and differential diagnosis of hirsutism.
 
PRINCIPLE OF THE TEST
The DHEA-S ELISA kit is based on the competition principle and the microplate separation. An unknown amount of DHEA-S present in the sample and a fixed amount of DHEA-S conjugated with horse-radish peroxidase compete for the binding sites of a polyclonal DHEA-S antiserum coated onto the wells. After one hour incubation the microtiterplate is washed to stop the competition reaction. Having added the substrate solution the concentration of DHEA-S is inversely proportional to the optical density measured.